Part:BBa_K1760000:Design

TodE

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal suffix found in sequence at 1
  Illegal EcoRI site found at 2049
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2049
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2049
  Illegal XhoI site found at 1033
  Illegal XhoI site found at 1925
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal suffix found in sequence at 2
  Illegal EcoRI site found at 2049
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2049
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 3055
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Designed biobrick is a composite part that contains a TetO promoter, RBS optimized by using Salis RBS calculator and codon optimized todE gene for E.Coli. TodE gene is part of a tod operon found in Pseudomonas Putida F1 species. It plays a major role in degradation of toluene. Specifically, todE degrades 3-methylcatechol, a highly toxic intermediate into HOD. In pseudomonas putida species, HOD is aerobically metabolized through TCA cycle. Theoretically, a E.Coli MG1655 type bacteria containing todC1C2BADEF genes can degrade toluene through TCA cycle by using its own native enzymes.

Source

Pseudomonas putida F1 (Synthesized)